We work closely with our clients to develop and validate ELISA or MSD assays in-house according to GLP specifications for Pharmacokinetic or Biomarker studies. We also work to validate commercially available ELISA or MSD assays.

We have experience working with a range of tissues (liver, kidney, brain, skin, retina, etc) and body fluids (serum, plasma, CSF, vitreous humor, etc)

We have experience in profiling compounds in a high-throughput manner. Some of the functional assays include cell viability, NanoBRET and apoptosis readouts. Mechanism of action assays include Gene (mRNA), Protein and Cytokine quantitation. The techniques used are In-Cell Westerns, Western Blots, Luminex, MSD, and qPCR. We have a collection of about 150 cell lines.

Shakti Bioresearch has worked closely with clients to establish stable cell lines and validate the biology of these cells via a range of experiments, from gene and protein expression to phenotypic analysis. We use a range of methods to develop stable cell lines, using plasmid and/or viral transfections.

We work closely with clients to express and purify recombinant proteins in bacterial and mammalian cells. We also have the capability to purify antibodies from hybridomas.

We have molecular biology capabilities, including cloning of genes into bacterial and viral vectors, site directed mutagenesis, cDNA library construction, siRNA knockdowns, and genomic DNA and RNA isolation.